PredIDR2: Improving accuracy of protein intrinsic disorder prediction by updating deep convolutional neural network...

Intrinsically disordered proteins (IDPs) or regions (IDRs) are widespread in proteomes, and involved in several important biological processes and implicated in many diseases. Many computational methods for IDR prediction are being developed to decrease the gap between the low speed of experimental determination of annotated proteins and the rapid increase of non-annotated proteins, and their performances are blindly tested by the community-driven experiment, the Critical Assessment of protein Intrinsic Disorder (CAID). In this paper, we developed PredIDR2 series, an updated version of PredIDR tested in CAID2 in order to accurately predict intrinsically disordered regions from protein sequence. It includes four methods depending on the input features and the producing mode of the negative samples of the training set. PredIDR2 series (AUC_ROC = 0.952) perform remarkably better than our previous PredIDR (AUC_ROC = 0.933) for Disorder-PDB dataset of CAID2, which seems to be mainly attributed to the introduction of a new deep convolutional neural network and the augmentation of the training data, especially from DisProt database. PredIDR2 series outperform the state-of-the-art IDR prediction methods participated in CAID2 in terms of AUC_ROC, AUC_PR and DC_mae and belong to the seven top-performing methods in terms of MCC. PredIDR2 series can be freely used through the CAID Prediction Portal available at https://caid.idpcentral.org/portal or downloaded as a Singularity container from https://biocomputingup.it/shared/caid-predictors/.