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Tumor Necrosis Factor-α, Vascular Endothelial Growth Factor and miRNA-145 Expression in Patients With Different
A O Yusupova1, O A Slepova1, N N Pakhtusov1
1Sechenov First Moscow State Medical University, Moscow.
Insights
MicroRNA-145 (miR-145) expression is significantly higher in patients with non-obstructive coronary artery disease (NOCA) compared to obstructive disease. Elevated miR-145 levels may indicate the presence of the NOCA phenotype in ischemic heart disease patients.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Biomarker Discovery
Background:
- Ischemic heart disease (IHD) encompasses various coronary artery disease (CAD) phenotypes, including obstructive and non-obstructive coronary artery disease (NOCA).
- Understanding the molecular differences between these phenotypes is crucial for accurate diagnosis and management.
- MicroRNA-145 (miR-145), tumor necrosis factor α (TNF-α), and vascular endothelial growth factor (VEGF) are implicated in cardiovascular processes.
Purpose of the Study:
- To evaluate and compare the expression levels of miR-145, TNF-α, and VEGF in patients with stable IHD presenting with either obstructive coronary artery (OCA) or non-obstructive coronary artery (NOCA) disease.
- To investigate the correlations between these biomarkers within each CAD phenotype.
- To assess the diagnostic potential of miR-145 in differentiating between NOCA and OCA phenotypes.
Main Methods:
- A cross-sectional observational study involving 107 patients (aged 45-75) with stable IHD.
- Patients were categorized into NOCA (51 patients) and OCA (56 patients) groups based on coronary angiography or CT data.
- Concentrations of VEGF, TNF-α, and miR-145 expression were measured and analyzed using statistical methods, including logistic regression and ROC analysis.
Main Results:
- Significantly higher concentrations of VEGF, TNF-α, and miR-145 expression were observed in the NOCA group compared to the OCA group.
- In the NOCA group, miR-145 expression correlated positively with VEGF and negatively with TNF-α.
- Logistic regression identified miR-145 expression and female gender as significant factors for CAD type. ROC analysis indicated miR-145 expression > 1.084 REU as a potential indicator for the NOCA phenotype.
Conclusions:
- miR-145 expression is significantly elevated in patients with the NOCA phenotype of stable IHD.
- A miR-145 expression level above 1.084 REU may serve as a diagnostic marker for identifying the NOCA phenotype in IHD patients.
- Lower miR-145 expression levels could be associated with more severe atherosclerotic CAD.
Abstract:
Aim To evaluate the expression level of miR-145, tumor necrosis factor α (TNF-α), and vascular endothelial growth factor (VEGF) in patients with ischemic heart disease (IHD) and different, obstructive and non-obstructive, phenotypes of coronary artery disease (CAD).Material and methods This cross-sectional observational study included 107 patients aged 45-75 years with a verified diagnosis of stable IHD. Based on the data of coronary angiography or spiral multislice computed tomography of CA, the patients were divided into two groups, with no obstructive (NOCA, 51 patients) and with obstructive coronary artery (OCA, 56 patients). In the NOCA group, women predominated (62.5%), while among patients with OCA, men predominated (67.9%). Concentrations of VEGF and TNF-α and miRNA-145 expression were measured in patients of both groups.Results In the NOCA group, the concentrations of VEGF (p=0.004) and TNF-α (p=0.002) and the miRNA-145 expression (p=0.014) were significantly higher. In this group, the miRNA-145 concentration was correlated with the concentrations of VEGF (ρ=0.442; p=0.013) and TNF-α (ρ=-0.386; p=0.032). In the OCA group, correlations were found between the concentrations of TNF-α and VEGF (ρ=0.645; p<0.001), miRNA-145 and VEGF (ρ=0.584; p<0.001), and miRNA-145c and TNF-α (ρ=0.421; p<0.001). According to the univariate logistic regression analysis, significant factors for the CAD type were miRNA-145 expression and female gender. A statistically significant ROC curve was constructed to evaluate the diagnostic capability of miRNA-145.Conclusion According to the study results, the highest level of miRNA-145 expression was found in the NOCA group. The ROC analysis showed that a level of miRNA-145 expression higher than 1.084 REU can be a factor of the NOCA phenotype presence in patients with stable IHD. A lower level of miRNA-145 expression can be associated with more severe atherosclerotic CAD.
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