Inhibition of sexual receptivity by anesthesia during estrogen priming

Anesthetics such as pentobarbital have been used to infer a role of neural activity in several neuroendocrine responses. The present experiments tested the hypothesis that neural activity during estrogen priming is necessary for the induction of sexual receptivity. Sexual behavior was induced in ovariectomized female rats by two pulses of unesterified estradiol 6 h apart, followed by progesterone at 19 h and testing at 24 h. Anesthetizing animals for approximately 1 h during each exposure to estradiol inhibited the induction of receptivity. Anesthesia immediately before each exposure to estradiol did not interfere with the induction of sexual receptivity. The induction of neural progestin receptors by estradiol was not prevented by anesthesia. Anesthesia had no effect on the uterine weight response to estradiol. These results suggest that neural activity during estrogen priming is required for the induction of sexual receptivity, and point to differences in the mechanism of action of estrogens in the brain and the uterus.