A novel study on enhancing ascorbic acid colorimetric detection: Green synthesis-driven crystallinity, stability, and catalytic performance of iron oxide nanoparticles in Mo(VI)/FeNPs-based biosensors

Kaouthar Ahmouda ¹, Mohammed Tayeb Oucif Khaled ¹

⁰Department of Process Engineering and Petrochemistry, Faculty of Technology, University of El Oued, El Oued, 39000, Algeria; Renewable Energy Research Unit in Arid Zones, University of El Oued, El Oued, 39000, Algeria.

Plant Physiology and Biochemistry : Ppb +

|

March 8, 2025

View abstract on PubMed

Summary

Green synthesis of iron oxide nanoparticles (FeNPs) using high-antioxidant plant extracts enhances their stability and catalytic activity. This improves Mo(VI)/FeNPs biosensors for sensitive ascorbic acid detection, lowering detection limits.

Area Of Science

Nanomaterials Science
Biosensor Technology
Green Chemistry

Background

Iron oxide nanoparticles (FeNPs) are crucial in biosensing applications.
Green synthesis offers an environmentally friendly route for FeNP production.
Optimizing FeNP properties is key to enhancing biosensor performance.

Purpose Of The Study

To investigate the influence of green synthesis on FeNP characteristics.
To correlate plant extract antioxidant capacity (TAC) with FeNP properties.
To evaluate the impact on Mo(VI)/FeNPs biosensor performance for ascorbic acid detection.

Main Methods

Green synthesis of FeNPs using plant extracts with varying TAC.
Characterization of FeNPs using FTIR and XRD.
Fabrication and testing of Mo(VI)/FeNPs biosensors for ascorbic acid.
Statistical analysis (ANOVA, Pearson correlation) to determine relationships.

Main Results

FeNPs synthesized from high-TAC extracts (e.g., Rosmarinus officinalis) showed enhanced crystallinity, stability, and γ-Fe2O3 phase.
These FeNPs led to superior catalytic performance in Mo(VI)/FeNPs biosensors.
Lower limits of detection (LOD) and quantification (LOQ) were achieved with high-TAC derived FeNPs.
FTIR confirmed efficient ascorbic acid oxidation, correlated with FeNP properties.

Conclusions

Plant extract TAC significantly influences FeNP crystallinity, phase composition, and stability.
Enhanced FeNP properties directly translate to improved biosensor sensitivity and lower detection limits.
Green synthesis provides a tunable platform for developing high-performance FeNPs for biosensing.

Keywords:

Ascorbic acid Biosensors Catalytic performance Colorimetric detection Green synthesis Iron oxide NPs Total antioxidant capacity