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Updated: May 23, 2025
Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
Published on: December 13, 2013
Alternative protocol for the histological preparation of animal tissues
Diógenes Siqueira-Silva1, Jeane Rodrigues2, Raquel Dos Santos Braga3
1Research Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Av. dos Ipês, S/N, Marabá, PA Zip-code: 68507-590, Brazil; Neuroscience and Behavior Laboratory "Frederico Guilherme Graeff" (LANEC), Psychology University, Institute of Healthy and Biologics Studies, Federal University of South and Southern of Pará, Av. dos Ipês, S/N, Marabá, PA 68507-590, Brazil; Postgraduation Program in Animal Reproduction in the Amazon (ReproAmazon) of the Federal Rural University of the Amazon (Ufra) and Federal University of Pará (UFPA), Av. Presidente Tancredo Neves, Nº 2501, Terra Firme, Belém, PA Zip-code: 66 077-830, Brazil; Graduate Program in Biodiversity and Biotechnology (BIONORTE) of the Federal University of Pará (UFPA), Rua Augusto Corrêa, 01 - Bairro Guamá, Belém, PA Zip-code: 66075-110, Brazil.
Abstract:
This study presents an alternative protocol that employs low-cost options to produce high-quality histology slides. The method is easily reproducible using recyclable materials, such as discarded blister packages, and simple adaptations, such as substituting a digital microscopy camera for a smartphone camera. Additionally, we provide a reliable method for setting image scale bars using office software and the open-access software ImageJ/Fiji. In summary, this protocol enables anyone to perform high-quality histology in a simpler and more accessible way.
