Quantitative histologic assessment of atrial fibrillation-associated fibrosis in animal models: A systematic review

Atrial fibrillation (AF) is the most common sustained arrhythmia, and cardiac fibrosis is a major component in driving its progressive nature. Quantitative histologic assessment of fibrosis in animal models is crucial for understanding AF, but current published studies present various methodologies that limit comparison. This systematic review examines 195 AF studies across multiple animal models (mice, rats, goats, dogs, pigs, and horses) to summarize (1) quantified fibrosis results and (2) methodologies for histologic fibrosis assessment; and (3) evaluate antifibrotic therapies used in these studies. The fibrosis quantified across the studies ranged from 0.34%-60.2% depending on the animal, intervention model, and quantification method. The findings underscore the need for a standardized fibrosis quantification protocol in AF research, enabling comparison across studies and offering greater insight into potential pharmacologic interventions.