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Updated: May 23, 2025
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
Published on: October 21, 2022
Down-regulated circ_0001853 inhibits lipopolysaccharide-induced endometritis progression via sponging miR-34c-5p
Qian Xu1, Ailing Peng1, Liyun Zhao1
1Department of Gynaecology, Changde Hospital, Xiangya School of Medicine, Central South University (The First People's Hospital of Changde City), Changde, 415003, China.
Insights
Circular RNA circ_0001853 and microRNA miR-34c-5p show diagnostic value for endometritis. Low circ_0001853 levels promote cell proliferation and inflammation by inhibiting miR-34c-5p, impacting disease progression.
Area of Science:
- Molecular Biology
- Gynecology
- Biochemistry
Background:
- Endometritis poses a significant health concern.
- Understanding the molecular mechanisms underlying endometritis is crucial for diagnosis and treatment.
- Circular RNAs and microRNAs are increasingly recognized as key regulators in various diseases.
Purpose of the Study:
- To investigate the diagnostic value of circ_0001853 and miR-34c-5p in endometritis.
- To elucidate the underlying mechanism of circ_0001853 and miR-34c-5p in endometritis progression.
Main Methods:
- Serum samples from 189 endometritis patients and 176 healthy controls were analyzed.
- Real-time quantitative PCR (RT-qPCR) was used to measure circ_0001853 and miR-34c-5p expression.
- Cell proliferation, apoptosis, inflammatory factors, and targeting interactions were assessed using CCK8, flow cytometry, ELISA, dual luciferase reporter assays, and RIP.
Main Results:
- Endometritis patients exhibited high circ_0001853 and low miR-34c-5p levels, showing a negative correlation.
- Both circ_0001853 and miR-34c-5p demonstrated diagnostic value for endometritis.
- Silencing circ_0001853 promoted cell proliferation, reduced apoptosis, and decreased inflammation, while miR-34c-5p inhibition reversed these effects.
Conclusions:
- circ_0001853 plays a role in endometritis progression via its interaction with miR-34c-5p.
- Low circ_0001853 levels appear to promote epithelial cell proliferation and suppress apoptosis and inflammation, contributing to disease development.
Background:
To investigate the diagnostic value and mechanism of action of circular RNA (circ_) circ_0001853 and microRNA (miR) miR-34c-5p in patients with endometritis.
Methods:
189 patients with endometritis and 176 healthy individuals were included in this study. Venous blood serum was collected from the study subjects and stored temporarily at -80 °C. Real-time quantitative chain polymerase reaction (RT-qPCR) was used to detect circ_0001853 and miR-34c-5p expression, and receiver operating characteristic (ROC) curves assessed the diagnostic value of both in predicting endometritis. Cell counting kit (CCK8) observed cell proliferation, flow cytometry recorded apoptosis, enzyme linked immunosorbent assay (ELISA) monitored inflammatory factor expression, and dual luciferase reporter assay and RNA immunoprecipitation (RIP) verified the relationship between circ_0001853 and miR-34c-5p targeting interactions.
Results:
High levels of circ_0001853 and low levels of miR-34c-5p were present in endometritis patients, and they were negatively correlated. Both circ_0001853 and miR-34c-5p alone or in combination had diagnostic value in predicting the progression of endometritis. Transfection of si-circ_0001853 promoted cell proliferation and reduced apoptosis and cellular inflammation levels induced by lipopolysaccharide (LPS) stimulation. There was a direct reciprocal targeting relationship between miR-34c-5p and circ_0001853, and the use of miR-34c-5p inhibitor resisted silencing circ_0001853 promoted cell proliferation and increased the number of apoptotic cells and cellular inflammation levels.
Conclusions:
circ_0001853 is involved in endometritis progression through miR-34c-5p, i.e., low circ_0001853 promotes miR-34c-5p-induced proliferation of epithelial cells, reduces apoptosis, and suppresses inflammation levels, preventing disease progression.
