Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Oxidative Stress Imbalance in Children and Adults with IBD and Associated Factors.

Nutrients·2026

Same author

Mitochondrial dysfunction in cerebrovascular diseases.

Trends in molecular medicine·2026

Same author

Occurrence of oxazolidinone resistance genes in enterococci, staphylococci, and Mammaliicoccus sciuri from swine slaughterhouse wastewater, Italy.

World journal of microbiology & biotechnology·2026

Same author

Hemp Essential Oils as Novel Antioxidant and Bacteriostatic Agents in PLA-Based Packaging.

Polymers·2026

Same author

Tn7860, a novel chromosomal pseudo-compound transposon carrying the OptrA gene in Enterococcus faecalis.

The Journal of antimicrobial chemotherapy·2026

Same author

Unveiling the toxic effects of perfluorooctanoic acid on osteoblast function and extracellular matrix deposition using 2D and 3D models.

Cell death discovery·2026

Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026

Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026

Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026

Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026

Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026

Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 23, 2025

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

Monitoring cellular dynamics upon infection using a holotomography-based approach.

Ilaria Nunzi¹, Gloria D'Achille², Nada Dhaouadi¹

¹Department of Clinical and Molecular Sciences, Marche Polytechnic University, Ancona, Italy.

Methods in Cell Biology

|March 9, 2025

Summary

This summary is machine-generated.

This study introduces holotomographic microscopy to track Listeria monocytogenes infection dynamics and cellular changes in real-time. This method visualizes bacterial movement and host cell remodeling without phototoxicity.

Keywords:

Bacterial infection Cell death Holotomography Listeria monocytogenes Mitochondria

More Related Videos

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Related Experiment Videos

Last Updated: May 23, 2025

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

Cellular microbiology
Microscopy techniques
Infectious disease dynamics

Background:

Intracellular bacteria manipulate host organelles, influencing infection spread and host defense.
Understanding pathogen-host interactions requires simultaneous monitoring of bacterial and cellular dynamics.
Holotomographic microscopy offers non-invasive, high-resolution live-cell imaging.

Purpose of the Study:

To develop and validate a holotomography-based method for observing intracellular bacterial dynamics.
To visualize the morphological effects of Listeria monocytogenes infection on host cells.
To provide a tool for studying pathogen-host interactions in real-time.

Main Methods:

Utilized holotomographic microscopy for live-cell imaging.
Tracked the migration of Listeria monocytogenes within host cells.
Monitored host cell morphological alterations induced by the infection.
Ensured extended observation periods with minimal phototoxicity.

Main Results:

Successfully detected Listeria monocytogenes dynamics using holotomography.
Visualized pathogen-mediated remodeling of the host cellular system at a morphological level.
Demonstrated the capability of holotomography for long-term, non-invasive infection monitoring.

Conclusions:

Holotomographic microscopy is a powerful tool for studying intracellular bacterial infections.
This approach enables comprehensive analysis of pathogen dynamics and host cell responses.
The method facilitates understanding of infection sequences and pathogen-induced cellular changes.