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Bioplastics

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

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Updated: Apr 6, 2026

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Controlling Protein Immobilization over Poly(3-hydroxybutyrate) Microparticles Using Substrate Binding Domain from

Isabela P Dias¹, Regiane Stafim da Cunha², Ryu Masaki¹

¹Department of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.

Biomacromolecules

|March 10, 2025

Summary

Researchers developed hybrid proteins to improve ligand attachment on polyester surfaces for targeted drug delivery. These novel SBD-tagged proteins enhanced binding and maintained orientation, showing promise for nanomedicine applications.

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Area of Science:

Biomaterials Science
Protein Engineering
Surface Chemistry

Background:

Biointerface ligand decoration is essential for controlling nanoparticle biodistribution and enabling targeted delivery.
Existing methods for functionalizing 3D polyester surfaces with ligands face challenges in efficiency and spatial orientation control.

Purpose of the Study:

To develop and characterize hybrid proteins with enhanced anchoring efficiency and controlled spatial orientation on polyester surfaces.
To evaluate the binding affinity of these novel proteins on poly(3-hydroxybutyrate) (PHB) microparticles and 2D surfaces.

Main Methods:

Engineered hybrid proteins by fusing reporter proteins (sfGFP, mRFP1) and a receptor-binding domain (RBD) to a polyester substrate-binding domain (SBD).
Assessed protein binding to poly(3-hydroxybutyrate) (PHB) microparticles and 2D surfaces using techniques to quantify protein content.
Evaluated the internalization and cytotoxicity of RBD-SBD decorated microparticles in MRC5 cells.

Main Results:

Proteins fused with SBD demonstrated significantly higher binding affinity and content on PHB interfaces compared to untagged proteins.
The SBD tag effectively enhanced protein immobilization on both microparticle and 2D polyester surfaces.
RBD-SBD decorated microparticles showed limited internalization and cytotoxicity in MRC5 cells.

Conclusions:

The developed SBD-based protein system offers a robust strategy for efficient and oriented ligand functionalization of polyester biointerfaces.
This approach holds significant potential for applications in targeted drug delivery and vaccine development.
The system minimizes unwanted cellular interactions, paving the way for safer nanomedicine formulations.