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Updated: May 23, 2025

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Yi Liu1, Kaikun Xu1, Cheng Chang1
1State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China.
Retention time (RT) alignment is crucial for correlating components in mass spectrometry (MS) based proteomics and metabolomics. This review categorizes RT alignment methods, discusses their applications, and explores future directions.
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