Related Experiment Video
Updated: May 23, 2025
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
In Vitro Plasma Protein Binding Determination of Lefamulin by Equilibrium Dialysis and High-Performance Liquid
Lin Xin1, Hong-Liang Xiao1, Mei-Di Li2
1College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics, Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
Abstract:
A credible method utilizing equilibrium dialysis and high-performance liquid chromatography with an evaporative light scattering detector (HPLC-ELSD) was developed to determine the plasma protein binding of lefamulin in swine. The method demonstrated excellent linearity, with a logarithmic calibration curve in the range of 0.1 to 2 μg/mL (r > 0.999). Extraction recoveries from plasma samples ranged from 81.0% to 90.8%, with coefficients of variation less than 7.21%. The limits of detection and quantitation were established at 0.05 and 0.1 μg/mL, respectively. The mean plasma protein binding rates of lefamulin were found to be 70.2 ± 8.0%, 68.4 ± 4.9%, and 68.4 ± 3.6%, respectively, with coefficients of variation below 11.4%. This method was satisfactorily applied to the determination of plasma protein binding rate of lefamulin with high precision, accuracy, and sensitivity.
