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ESIPT-driven imaging: a thiazole probe for lipid droplets and plasma membranes
José L Belmonte-Vázquez1, Juan L Cortes-Muñoz2, Adriana Romo-Pérez2
1Departamento de Química Orgánica - Facultad de Química (FQ), Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Ciudad de México 04510, Mexico.
Chemical Communications (Cambridge, England)
|March 10, 2025
Summary
This study presents a new probe for fluorescence imaging that can track cellular structures. The probe initially binds to the plasma membrane before moving to lipid droplets.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Imaging
Background:
- Fluorescence imaging is crucial for visualizing cellular functions.
- Developing specific probes enhances the resolution and detail of cellular imaging.
Purpose of the Study:
- To introduce a novel Excited-State Intramolecular Proton Transfer (ESIPT) probe.
- To demonstrate the probe's dual localization capability for plasma membranes and lipid droplets.
Main Methods:
- Utilizing fluorescence imaging techniques.
- Designing and synthesizing a novel ESIPT probe.
- Observing probe localization and redistribution in cellular environments.
Main Results:
- The probe exhibits initial affinity for the plasma membrane, visualized via blue channel imaging.
- The probe subsequently redistributes to lipid droplets.
- The probe demonstrates strong affinity for the hydrophobic lipid core of lipid droplets.
Conclusions:
- The novel ESIPT probe offers dual localization for key cellular compartments.
- This probe enhances the visualization of lipid droplets and plasma membranes.
- The probe's behavior provides insights into cellular lipid dynamics.
