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Published on: January 16, 2015
Mass Spectrometric Identification of Urinary Biomarkers of Chronic Kidney Disease: A Proteomic-Related Preliminary
Sangeetha P Kademani1, Prabhudas Nelaturi1, Sathya Sagar Kalidas2
1Multi-Disciplinary Centre for Biomedical Research, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission's Research Foundation (Deemed to be University), Kirumampakkam, Puducherry, India.
Background:
Chronic kidney disease (CKD) is a gradual loss of kidney function and has an increased prevalence rate worldwide. Our study was intended to identify potential biomarkers of progression using urine proteomics.
Materials And Methods:
This preliminary study consisted of 32 patients with stage V CKD. Urine samples were subjected to liquid chromatography-mass spectrometry (LCMS), and the network of protein interaction was analyzed using STRING.
Results:
A total of 135 proteins were identified, of which 35 were listed as candidates based on their clinical significance. Protein- protein interaction study provides novel insights into the functional constitution of the proteome, selecting urine as a source of biomarkers.
Conclusion:
The present study observed that the potential markers such as EndoG, HPX, APN, AnxA1, Mic60, LONP1, and HYOU1 correlate with renal damage and its progression to CKD stage V.
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