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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jul 9, 2026

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Current Issues of Next-Generation Sequencing-Based Circulating Tumor DNA Analysis in Colorectal Cancer.

Narmadha Rathnasamy¹, Viraj Lavingia², Shyam Aggarwal³

¹Department of Medical Oncology, Royal Care Superspeciality Hospital, Coimbatore, Tamil Nadu, India.

South Asian Journal of Cancer

|March 10, 2025

Summary

This summary is machine-generated.

Circulating tumor deoxyribonucleic acid (ctDNA) offers a noninvasive method for cancer monitoring. This review highlights its increasing utility in managing colorectal cancer patients using next-generation sequencing techniques.

Keywords:

molecular monitoring progression residual disease targeted

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Last Updated: Jul 9, 2026

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Area of Science:

Oncology
Molecular Biology
Genomics

Background:

Circulating tumor deoxyribonucleic acid (ctDNA) is increasingly recognized for its potential in cancer management.
Noninvasive testing methods for ctDNA are becoming more accurate and frequent.
The application of ctDNA in patient monitoring, especially for colorectal cancer, is expanding.

Purpose of the Study:

To review the current role of circulating tumor deoxyribonucleic acid (ctDNA) in cancer monitoring.
To focus on ctDNA measurement using next-generation sequencing (NGS)-based methods.
To discuss the clinical utility of ctDNA in colorectal cancer management.

Main Methods:

Literature review of studies employing next-generation sequencing for ctDNA analysis.
Analysis of ctDNA detection accuracy, frequency, and noninvasive applications.
Evaluation of ctDNA's role in monitoring cancer patients, with emphasis on colorectal cancer.

Main Results:

Evidence supports the accurate and frequent noninvasive testing of ctDNA.
The use of ctDNA for monitoring cancer patients, particularly those with colorectal cancer, is growing.
Next-generation sequencing-based methods are key to current ctDNA applications.

Conclusions:

Circulating tumor deoxyribonucleic acid (ctDNA) is a valuable noninvasive biomarker for cancer monitoring.
Next-generation sequencing enables accurate and frequent ctDNA analysis.
ctDNA shows significant promise in the management of colorectal cancer.