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Updated: Apr 25, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
TEMI: Tissue Expansion Mass Spectrometry Imaging.
We developed Tissue Expansion compatible with Mass spectrometry Imaging (TEMI) for high-resolution biomolecule mapping. TEMI reveals metabolic heterogeneity in tumors and advances spatial multi-omics research.
Area of Science:
- Biochemistry
- Molecular Biology
- Biophysics
Background:
- Spatial distribution of biomolecules is vital for organism function.
- High-throughput in situ mapping requires speed, resolution, and sensitivity.
Purpose of the Study:
- To develop a novel method for high-throughput spatial biomolecule mapping.
- To achieve single-cell resolution without compromising voxel throughput.
Main Methods:
- Developed Tissue Expansion compatible with matrix-assisted laser desorption/ionization Mass spectrometry Imaging (TEMI).
- Applied TEMI to profile lipids, metabolites, peptides, and N-glycans.
Main Results:
- Achieved single-cell spatial resolution for biomolecule profiling.
- Mapped biomolecule distribution in mammalian tissues.
- Uncovered metabolic heterogeneity within tumors.
Conclusions:
- TEMI offers high-throughput, high-resolution spatial multi-omics profiling.
- TEMI is broadly applicable in biological and medical research.
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