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Updated: May 23, 2025

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
Published on: July 8, 2021
Lactate metabolic coupling between the endplates and nucleus pulposus via MCT1 is essential for intervertebral disc
Abstract:
During skeletal growth, there is an increased secretion of lactate by glycolytic nucleus pulposus (NP) cells of the intervertebral disc. To investigate the role of this anion, we generated annulus fibrosus (AF) and endplate (EP) specific Mct1 ( Slc16a1 ) mice. Histological and spatial transcriptomic studies indicated significant disc degeneration in Mct1 , characterized by NP cell loss and delayed EP maturation. Metabolic assays showed that while AF and EP cells were glycolytic, EP chondrocytes readily metabolized lactate. In EP cells, lactate promoted protein, and H3K18 lactylation, implying epigenetic programming. These findings suggest that NP-derived lactate promotes EP cartilage transdifferentiation into the subchondral bone, and in its absence, continued glucose consumption by the persistent EP cartilage reduces glucose availability to the NP and AF likely contributing to tissue degeneration. This study provides the first in vivo evidence that metabolic coupling between NP and EP cells is essential for disc growth and health.
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