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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

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Luminos: open-source software for bidirectional microscopy.

Daniel G Itkis¹, F Phil Brooks¹, Hunter C Davis¹

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge MA 02138.

Biorxiv : the Preprint Server for Biology

|March 10, 2025

Summary

This summary is machine-generated.

Bidirectional microscopy (BDM) requires precise experimental alignment. Luminos is an open-source MATLAB library offering microsecond-accurate synchronization for BDM, enhancing biological research.

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Area of Science:

Biophysics
Cell Biology
Neuroscience

Background:

Bidirectional microscopy (BDM) enables simultaneous optical perturbation and signal imaging.
Precise spatial and temporal alignment of stimulation and recording is crucial for BDM.
Existing methods face challenges in achieving high-accuracy synchronization.

Purpose of the Study:

To introduce Luminos, an open-source MATLAB library for controlling BDM experiments.
To provide modular and precisely synchronized control for BDM.
To facilitate the development and application of BDM techniques.

Main Methods:

Developed an open-source MATLAB library named Luminos.
Implemented hardware-triggered synchronization for stimulation, recording, and imaging channels.
Achieved microsecond-level timing accuracy.

Main Results:

Luminos offers modular control for BDM experiments.
The library ensures precise hardware-triggered synchronization with microsecond accuracy.
Open-source code and documentation are publicly available.

Conclusions:

Luminos provides a robust solution for synchronizing BDM experiments.
This tool will accelerate advancements in bidirectional microscopy across biological sciences.
The library's availability promotes wider adoption and development of BDM methods.