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Related Experiment Video

Updated: May 23, 2025

Fecal micro RNA Isolation

Fecal micro RNA Isolation

Published on: October 28, 2020

A protocol for microRNA extraction from gastrointestinal digesta.

Miguel Cifuentes Acebal^1,2, Yvan Devaux³, Torsten Bohn¹

¹Nutrition and Health Research Group, Department of Precision Health, Luxembourg Institute of Health, Strassen, Luxembourg.

Food Chemistry. Molecular Sciences

|March 10, 2025

Summary

Diet-derived microRNAs (miRNAs) are challenging to study due to gastrointestinal digestion. This new silica column method efficiently extracts and quantifies these dietary miRNAs, enabling their use as intake biomarkers.

Keywords:

Digestive enzymes INFOGEST In vitro digestion Intestine Nucleotides XenomiR

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Last Updated: May 23, 2025

Fecal micro RNA Isolation

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Published on: October 28, 2020

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Area of Science:

Biochemistry
Molecular Biology
Nutrition Science

Background:

MicroRNAs (miRNAs) are non-coding RNAs regulating gene expression.
Diet-derived miRNAs can survive ingestion, but their study is hindered by gastrointestinal digestion.
Challenges include chemical interference and high RNAse activity in the gut matrix.

Purpose of the Study:

To develop a method for extracting and quantifying diet-derived miRNAs from gastrointestinal digesta.
To overcome limitations posed by RNAse activity, bile salts, and plant bioactive substances.

Main Methods:

Silica column purification technique applied to the bioaccessible phase of gastrointestinal digesta.
Method optimized to address challenges of RNAse activity and matrix constituents.

Main Results:

A simple, rapid (under 2 hours), reliable, and systematic protocol for miRNA purification was established.
The method successfully overcomes inhibitory factors present in gastrointestinal digesta.
Enables the study of the miRNA fraction in a previously neglected body fluid.

Conclusions:

The developed method facilitates the study of diet-derived miRNAs in gastrointestinal digesta.
Diet-derived miRNAs can potentially serve as biomarkers for dietary intake and biological changes.
Opens new avenues for research into the role of dietary miRNAs in health and metabolism.