Related Experiment Video
Updated: May 23, 2025
Dissection of the Endolymphatic Sac from Mice
Published on: March 29, 2021
Microscale Proteomic Analysis of the Endolymphatic Sac in Menière's Disease Patients
Wenting Deng1, Yuelian Luo1, Haoyang Wu1
1Department of Otolaryngology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
Objective:
Endolymphatic hydrops is the primary pathological feature of Menière's disease (MD). An imbalance between endolymphatic fluid production and absorption due to endolymphatic sac (ES) dysfunction may be the major cause of endolymphatic hydrops. This study aimed to identify and characterize global protein expression of the ES in MD patients using microscale proteomics for the first time.
Methods:
Liquid chromatography-tandem mass spectrometry was used to analyze the ES samples from six MD patients and six acoustic neuroma (AN) patients (as control) for microscale proteomics and differentially expressed proteins (DEPs) were screened.
Results:
A total of 1,213 proteins in the ES were identified. Among them, there were 40 DEPs between the two groups. Of these proteins, 34 were upregulated (e.g., DPT and CHMP1B), and 6 were downregulated (e.g., PLPP3 and SCARB2) in the MD group compared to the AN group. DEPs were significantly enriched in coagulation and complement cascades.
Conclusion:
Microscale proteomics revealed a unique protein expression pattern in the ES of MD patients. Several proteins such as DPT, CHMP1B, and LTA4H may be involved in the pathogenesis of MD.
Insights
Microscale proteomics revealed unique protein patterns in the endolymphatic sac (ES) of Meniere's disease (MD) patients. These findings offer new insights into the molecular mechanisms underlying MD pathogenesis.
Area of Science:
- Otorhinolaryngology
- Proteomics
- Molecular Biology
Background:
- Endolymphatic hydrops, a hallmark of Meniere's disease (MD), is linked to endolymphatic sac (ES) dysfunction.
- Dysfunction in the ES may disrupt fluid balance, leading to hydrops.
Purpose of the Study:
- To identify and characterize the global protein expression profile of the ES in MD patients.
- To investigate the role of ES protein alterations in the pathogenesis of Meniere's disease using microscale proteomics.
Main Methods:
- Microscale proteomics was employed to analyze ES samples.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for protein identification and quantification.
- ES samples from six MD patients and six acoustic neuroma (AN) patients (controls) were analyzed.
Main Results:
- A total of 1,213 proteins were identified in the ES.
- Forty differentially expressed proteins (DEPs) were found between MD and AN groups.
- Proteins involved in coagulation and complement cascades were significantly enriched in MD patients, with notable upregulation of DPT and CHMP1B, and downregulation of PLPP3 and SCARB2.
Conclusions:
- Microscale proteomics identified a distinct protein expression signature in the ES of MD patients.
- Specific proteins, including DPT, CHMP1B, and LTA4H, are implicated as potential contributors to MD pathogenesis.
- These findings advance the understanding of molecular mechanisms in Meniere's disease.
