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Application of versatile reverse genetics system for feline coronavirus
Izumi Kida1, Tomokazu Tamura2,3,4, Yudai Kuroda5
1Division of Risk Analysis and Management, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Microbiology Spectrum
|March 10, 2025
Summary
Researchers developed a new reverse genetics system for feline coronavirus (FCoV), enabling the study of feline infectious peritonitis (FIP) pathogenesis. This tool allows for genetic modifications and the development of antiviral strategies against FCoV.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Feline infectious peritonitis (FIP) is a fatal disease in cats caused by feline coronavirus (FCoV).
- Molecular studies of FCoV are hindered by the absence of a robust reverse genetics system.
Purpose of the Study:
- To establish a rapid and versatile reverse genetics system for FCoV.
- To enable genetic manipulation of FCoV for studying pathogenesis and developing antivirals.
Main Methods:
- Developed a PCR-based circular polymerase extension reaction (CPER) system for FCoV.
- Generated recombinant FCoV (serotypes 1 and 2) with high sequence accuracy.
- Introduced spike gene modifications and a HiBiT reporter tag into recombinant FCoV.
Main Results:
- Successfully rescued recombinant FCoV with comparable growth kinetics to parental strains.
- Demonstrated stable expression of the HiBiT tag in recombinant viruses after serial passages.
- Showcased the utility of reporter viruses for evaluating antiviral inhibitor sensitivity.
Conclusions:
- The developed reverse genetics system is a versatile tool for FCoV research.
- This system facilitates characterization of viral genes and investigation of FCoV proliferation and pathogenicity.
- The system aids in understanding FIP disease mechanisms and evaluating antiviral efficacy.
