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AC074117.1/miR-193a-3p axis regulates the malignant progression of uterine corpus endometrial carcinoma via the m6A-related gene ALKBH5

  • 0Department of Obstetrics and Gynecology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, Jiangsu Province, China; Department of Obstetrics and Gynecology,Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 201600, China.
The American Journal of the Medical Sciences +

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March 10, 2025

|

March 10, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identified a novel m6A-related ceRNA network involving AC074117.1, miR-193a-3p, and ALKBH5 that promotes uterine corpus endometrial carcinoma (UCEC) progression. This network offers potential targets for UCEC diagnosis and treatment.

Area Of Science

  • Oncology
  • Molecular Biology
  • Epigenetics

Background

  • Uterine corpus endometrial carcinoma (UCEC) is a prevalent gynecological malignancy with increasing incidence and poor prognosis.
  • Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are key regulators of UCEC via competing endogenous RNA (ceRNA) networks.
  • N6-methyladenosine (m6A) modification is implicated in UCEC progression, but its role in m6A-associated ceRNA networks remains unclear.

Purpose Of The Study

  • To elucidate the role of m6A-associated ceRNA networks in the malignant progression of UCEC.
  • To identify specific molecular players and their interactions within these networks.
  • To explore potential diagnostic and therapeutic targets for UCEC.

Main Methods

  • Bioinformatic analysis to construct the m6A-related ceRNA regulatory network in UCEC.
  • MeRIP assays to investigate m6A modification regulated by ALKBH5.
  • Dual-luciferase reporter assays to confirm the interaction between AC074117.1 and miR-193a-3p.
  • Cellular assays (proliferation, migration) and rescue experiments to validate the functional role of the identified axis.

Main Results

  • The AC074117.1/miR-193a-3p axis was identified to promote UCEC malignant progression by targeting ALKBH5, an m6A demethylase.
  • MeRIP assays confirmed ALKBH5's role in regulating m6A modification in UCEC.
  • The AC074117.1/miR-193a-3p/ALKBH5 axis was shown to regulate UCEC cell proliferation and migration.
  • Functional rescue experiments demonstrated that miR-193a-3p's effects on UCEC progression are partially dependent on ALKBH5.

Conclusions

  • This study reveals a novel m6A-related ceRNA network (AC074117.1/miR-193a-3p/ALKBH5) in UCEC.
  • This network plays a significant role in promoting UCEC malignant progression.
  • The identified m6A-related ceRNA network holds potential as a target for early diagnosis and treatment of UCEC.

Keywords:

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