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Updated: May 23, 2025

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Published on: December 18, 2013
Effect of glucose supplementation on protein biosynthesis in chickens reared under thermoneutral or heat stress
Josephine Kwakye1, Oluwatomide W Ariyo1, Ahmed F A Ghareeb1
1NutriGenomics Laboratory, Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
Abstract:
Heat stress (HS) impacts broilers by reducing feed intake which impairs nutrient availability and energy levels, subsequently affecting protein biosynthesis. We hypothesize that an exogenous supply of glucose could provide extra energy resources that enhance protein biosynthesis in broilers reared under HS. Our experimental design involved two levels of temperature (25 °C [thermoneutral, TN]); 35 °C (8.00 AM to 8.00 PM, [Heat Stress, HS]), and two glucose levels (0 % and 6 %). We randomly assigned a total of 456 four-week-old Cobb500 broilers to four different treatment groups (TN0, TN6, HS0, and HS6), respectively. After 7 days post-HS, we observed an inverse relationship between the avian target of rapamycin (avTOR) and autophagy-related genes. The phosphorylation of mTOR and S6K1 at Ser2448 and Thr421/Ser424 respectively was higher (p < 0.05) in the TN0 group than in the HS groups. Additionally, the phosphorylation of Foxo3a at Ser253 was higher (p < 0.05) in the HS0 group than in the HS6 groups, indicating an adaptive response to HS. Thus, the combined effect of HS and glucose could influence the phosphorylation status of key signaling genes in the mTOR pathway. The expression levels of mRNA genes in the mTOR pathway were more pronounced (p < 0.05) in HS6 birds than in HS0 birds except for avTOR, Akt1, and S6K1.
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