Related Experiment Video
Updated: May 23, 2025
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Molecular and genomic investigation unveils Pseudomonas putida as an emerging multidrug-resistant pathogen linked to
Tima Tisa Mallick1, Md Morshedur Rahman1, Naim Siddique1
1Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.
Abstract:
Pseudomonas putida is one of the emerging pathogens responsible causing mastitis in lactating animals. This study investigated the prevalence, antimicrobial resistance (AMR), genetic diversity and virulence factor genes (VFGs) to highlight the pathogenic potentials of P. putida strains isolated from milk, feces and farm soil of dairy cows diagnosed with clinical mastitis (CM). A total of 110 samples were collected and analyzed, revealing an overall prevalence of P. putida in dairy farms at 40.90 %, with specific prevalence rates of 42.22 % in milk, 26.67 % in feces, and 31.11 % in farm soil. In vitro antimicrobial assays demonstrated that 76.0 % P. putida isolates exhibited multidrug resistance (MDR, resistance to ≥ 3 antibiotics), particularly showing high resistance to oxacillin, ampicillin, nalidixic acid, and aztreonam. Conversely, P. putida isolates showed the highest susceptibility against imipenem. The genome analysis of three MDR P. putida strains 11CM-M1 (milk), 11CM-F1 (feces) and 11CM-S1 (farm soil), showed a close evolutionary relationship with different strains of Pseudomonas spp. isolated from bovine mastitis milk and feces samples, human stool, and samples sourced from hospital environment. The assembled genomes of three P. putida strains encoded nine antibiotic resistance genes (ARGs), 36 VFGs, and 367 metabolic subsystems, highlighting a complex functional profile and potential for pathogenicity. The detailed analysis of these ARGs and VFGs demonstrated that P. putida strains employ distinct mechanisms of resistance (e.g., efflux pumps), biofilm formation, and virulence factors, including adhesins, secreted toxins, and lipopolysaccharides, which contribute to their pathogenic potential. Given the lack of reports linking P. putida strains to bovine mastitis in Bangladesh, the increasing trend of AMR, along with the presence of significant ARGs and VFGs in the studied strains, underscores the need for more intensive research, including animal model experiment, to better elucidate the pathogenesis and inform treatment decisions for mastitis in dairy animals.
Insights
Pseudomonas putida is an emerging cause of bovine mastitis, showing high multidrug resistance (MDR) and carrying virulence genes. This highlights the need for further research into its pathogenicity and treatment strategies.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Bovine Health
Background:
- Pseudomonas putida is increasingly recognized as a pathogen causing mastitis in lactating animals.
- Understanding its prevalence, resistance, and virulence is crucial for managing bovine mastitis.
Purpose of the Study:
- To investigate the prevalence, antimicrobial resistance (AMR), genetic diversity, and virulence factor genes (VFGs) of P. putida in dairy farms.
- To assess the pathogenic potential of P. putida strains isolated from cows with clinical mastitis (CM).
Main Methods:
- Collected 110 samples (milk, feces, farm soil) from dairy cows with CM.
- Performed in vitro antimicrobial assays and genome analysis of selected P. putida strains.
- Identified antibiotic resistance genes (ARGs) and virulence factor genes (VFGs).
Main Results:
- P. putida prevalence was 40.90% overall (42.22% milk, 26.67% feces, 31.11% soil).
- 76.0% of isolates showed multidrug resistance (MDR), with high resistance to oxacillin, ampicillin, nalidixic acid, and aztreonam; high susceptibility to imipenem.
- Genome analysis revealed nine ARGs, 36 VFGs, and close evolutionary links to other Pseudomonas spp., indicating pathogenic potential via resistance mechanisms and virulence factors.
Conclusions:
- P. putida strains in Bangladesh dairy farms exhibit significant AMR and possess virulence factors, posing a threat for bovine mastitis.
- The findings underscore the urgent need for further research, including animal models, to understand pathogenesis and guide mastitis treatment.
- Genomic insights reveal complex resistance and virulence strategies employed by P. putida in dairy environments.
More Related Videos
04:37Author Spotlight: Understanding Rhamnolipid Regulation in Pseudomonas aeruginosa
Published on: March 29, 2024
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
