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Updated: May 23, 2025

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Tissue Printing Hybridization Using Digoxigenin-Labeled DNA Probes for the Detection of Geminiviruses and Associated
Elvira Fiallo-Olivé1, Jesús Navas-Castillo2
1Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Consejo Superior de Investigaciones Científicas, Algarrobo-Costa, Málaga, Spain. efiallo@eelm.csic.es.
Abstract:
Nucleic acid hybridization is a fundamental technique in plant virus research, enabling precise detection of plant pathogens. Tissue printing hybridization exhibits high sensitivity and specificity in detecting viral genomes, providing particularly valuable for screening a large number of samples without the need for DNA purification. This method virtually eliminates contamination and false positives. This chapter presents a detailed and effective protocol for nucleic acid hybridization of geminiviruses using digoxigenin-labeled probes. Specifically, the tissue printing hybridization technique is outlined for a particular geminivirus and its associated deltasatellite. However, the methodology is adaptable to a diverse range of geminiviruses and host plants, rendering it versatile and applicable across various geminivirus research contexts.
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