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Updated: May 23, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Chromosome-level genome assembly of Jaguar guapote (Parachromis manguensis) by massive parallel sequencing
Jianmeng Cao1, Yannan Tong2, Zhigang Xiao3
1Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China. caojianmeng@aliyun.com.
Abstract:
Parachromis managuensis is a native cichlid fish from Central America and is the most commonly traded species within its genus. This study presents the first chromosome-scale genome assembly of P. managuensis using PacBio HiFi long reads and Hi-C sequencing data. The size of the P. managuensis genome is approximately 896.66 Mb, with a scaffold N50 of 38.19 Mb. The assembled genome demonstrates high quality in terms of completeness and accuracy, with a BUSCO score of 98.85% and a quality value (QV) of 50.95. A total of 888.60 Mb (99.10%) sequences were anchored to 24 pseudochromosomes. Additionally, 21,145 protein-coding genes and 325.58 Mb (~36.31%) repetitive sequences were identified. This chromosome-level genome assembly provides a crucial reference for studying the evolution and ecological adaptability of P. managuensis.
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