Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

86.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
86.8K
Sanger Sequencing01:57

Sanger Sequencing

752.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.1K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
Complementary DNA01:44

Complementary DNA

29.2K
Overview
29.2K
Gene Conversion02:08

Gene Conversion

9.6K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of sleep variability and irregularity with gestational metabolic syndrome: a birth cohort study.

Frontiers in endocrinology·2026
Same author

Genetic analysis of biopsy-related droplets in patients undergoing PGT-A and its potential application.

Frontiers in endocrinology·2026
Same author

Gonadotropin-releasing hormone agonist (GnRH-a) pretreatment duration and letrozole supplementation for optimizing live birth rates in women with adenomyosis undergoing frozen-thawed embryo transfer (GOLD-FET): study protocol for a multicenter, 2 × 2 factorial randomized controlled trial in China.

Trials·2026
Same author

An NIR/GSH-responsive nanoplatform based on tetrasulfide bridging for targeted synergistic tumor therapy.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Clinical correlation of apoptosis in human luteinized granulosa cells.

Journal of ovarian research·2026
Same author

Vitamin D supplementation before in vitro fertilisation in women with polycystic ovary syndrome: multicentre, double blind, placebo controlled, randomised clinical trial.

BMJ (Clinical research ed.)·2026

Related Experiment Video

Updated: May 23, 2025

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.0K

An exogenous encoding sequence based on DNA data storage technology and its application in assisted reproductive

Zhiqing Huang1,2, Taoli Ding3, Zixuan Ni4

  • 1Center of Reproductive Medicine, Fujian Maternity and Child Health Hospital, Fuzhou, 350001, China.

Biochemistry and Biophysics Reports
|March 11, 2025
PubMed
Summary

A novel DNA data storage system uses exogenous encoding sequences (EES) to uniquely identify each in vitro cultured embryo. This method enhances sample traceability and prevents mix-ups in assisted reproductive technology (ART) and genetic testing labs.

Keywords:
Assisted reproductive technologyExogenous encoding sequenceNext-generation sequencingPreimplantation genetic testSafety

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
00:09

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.3K

Related Experiment Videos

Last Updated: May 23, 2025

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.0K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
00:09

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.3K

Area of Science:

  • Biotechnology
  • Genetics
  • Reproductive Medicine

Background:

  • Assisted reproductive technology (ART) faces safety and ethical challenges due to sample confusion and contamination in laboratories.
  • Current tracing systems are often inadequate for identifying the source of errors in high-volume PGT and third-party testing labs.
  • This poses risks to fertility centers, testing facilities, and patient families.

Purpose of the Study:

  • To develop an effective and feasible tracing system for in vitro cultured embryos.
  • To prevent sample confusion and contamination in ART and genetic testing.
  • To mitigate associated risks and ethical concerns.

Main Methods:

  • Designed an exogenous encoding sequence (EES) based on DNA data storage technology for unique embryo identification.
  • Verified EES amplification with embryo biopsy cells and detection via sequencing without impacting copy number variants (CNVs).
  • Assessed EES's ability to detect contamination exceeding 5%.

Main Results:

  • The EES provides a unique identifier for each embryo, effectively preventing mix-ups.
  • EES is non-toxic, stable, and amplifiable with biopsy cells.
  • The system reliably detects contamination from other samples.
  • EES does not interfere with CNV analysis.

Conclusions:

  • The EES is an effective and reliable tool for enhancing embryo safety in ART.
  • This DNA data storage-based system can be implemented in fertility and third-party labs.
  • It significantly reduces the risk of sample mix-ups and associated ethical issues.