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Related Experiment Video

Updated: May 8, 2025

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EntoSieve: Automated Size-Sorting of Insect Bulk Samples to Aid Accurate Megabarcoding and Metabarcoding.

Aleida Ascenzi1,2, Lorenz Wührl3, Vivian Feng4,5

  • 1Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, Rome, Italy.

Molecular Ecology Resources
|March 11, 2025
PubMed
Summary
This summary is machine-generated.

Insect monitoring requires efficient, cost-effective methods. The new EntoSieve device rapidly sorts insect samples by size, improving DNA-based biodiversity assessments and reducing contamination for megabarcoding and metabarcoding studies.

Keywords:
DNA barcodingbiomass biasbulk samplesinsect biodiversitysequence recovery

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Area of Science:

  • Ecology
  • Molecular Biology
  • Bioinformatics

Background:

  • Widespread insect decline necessitates standardized, cost-effective monitoring protocols.
  • Current molecular methods like metabarcoding face challenges with sample heterogeneity, particularly body size, impacting DNA-based diversity assessments.
  • Existing automated, rapid, non-destructive size-sorting solutions are lacking.

Purpose of the Study:

  • To introduce the EntoSieve, a low-cost, DIY motorized instrument for disentangling and size-sorting insect bulk samples.
  • To evaluate the efficiency and impact on specimen integrity of the EntoSieve.
  • To demonstrate the device's utility in preparing samples for DNA-based biodiversity analyses.

Main Methods:

  • Development of a low-cost, DIY motorized instrument (EntoSieve) using readily available components and 3D-printed parts.
  • Testing the EntoSieve on three insect bulk samples (>10,000 specimens) using three sieving protocols.
  • Assessment of sorting efficiency, processing time, and specimen damage.

Main Results:

  • EntoSieve achieved high sorting efficiencies (92%-99%) within 18-60 minutes.
  • Specimen damage was found to be not significant for subsamples.
  • The device effectively disentangles and sorts insects into size fractions, minimizing cross-contamination.

Conclusions:

  • The EntoSieve offers a rapid, cost-effective, and low-damage solution for insect sample pre-processing.
  • This device facilitates the production of morphologically valuable vouchers for megabarcoding.
  • EntoSieve is expected to improve the accuracy of compositional diversity assessments in metabarcoding across different insect size classes.