Related Experiment Video

Updated: Aug 12, 2026

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
13:22

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Published on: September 10, 2013

11.9K

False negative ELISA screen for benzoylecgonine in blood using the Neogen® assay

Jennifer F Limoges1, Seth J Tracy1, Heidi E Grieger1

  • 1Toxicology, New York State Police Forensic Investigation Center, Albany, New York, USA.

Journal of Forensic Sciences
|March 11, 2025
PubMed
Summary

Enzyme-linked immunosorbent assay (ELISA) screening for benzoylecgonine (BE) in blood can yield false negatives, impacting forensic toxicology. The Neogen® BE assay showed this anomaly, with the cause still undetermined.

Keywords:
ELISAbenzoylecgonineblood drug screencocainefalse negativeforensic toxicology

More Related Videos

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

14.6K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

2.4K

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.