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Updated: Aug 7, 2026
05:03
Establishing a Silicosis Rat Model via Exposure of Whole-Body to Respirable Silica
Published on: October 28, 2022
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Silicone Oil Affects Fibrosis of Human Trabecular Meshwork Cells by Upregulating Ferroptosis Through a ROS/NOX4/Smad3
Jing Wang1, Yang Zhang1, Huimin Zhong2
1Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China.
Investigative Ophthalmology & Visual Science
|March 11, 2025
Summary
Silicone oil (SiO) can cause secondary glaucoma by damaging trabecular meshwork cells. This study reveals ferroptosis and the ROS/NOX4/Smad3 pathway are key mechanisms, offering targets to prevent complications.
Area of Science:
- Ophthalmology
- Cell Biology
- Biochemistry
Background:
- Silicone oil (SiO) is crucial for retinal reattachment in complex cases.
- Secondary glaucoma is a significant complication of intravitreal SiO tamponade.
- Trabecular meshwork cells (HTMCs) are vital for regulating intraocular pressure.
Purpose of the Study:
- To investigate the effects of SiO on HTMCs.
- To elucidate the mechanisms underlying SiO-induced ocular complications.
- To identify potential therapeutic targets for mitigating SiO-related adverse effects.
Main Methods:
- Co-culture of HTMCs with SiO.
- Assessment of cell proliferation, fibrosis markers, and ferroptosis.
- Gene knockdown of NOX4 and Smad3 to explore underlying pathways.
Main Results:
- SiO inhibited HTMC proliferation and increased fibrosis markers.
- SiO elevated ferroptosis levels in HTMCs.
- NOX4 upregulation and Smad3 activation mediated SiO-induced ferroptosis and ROS increase.
Conclusions:
- Ferroptosis and the ROS/NOX4/Smad3 axis are critical in SiO-induced intraocular pressure elevation.
- These findings offer insights into potential therapeutic strategies.
- Mitigating postoperative complications associated with SiO tamponade is a key goal.
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