Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.4K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.4K
What is Gene Expression?01:36

What is Gene Expression?

8.3K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.3K
Renal Corpuscle01:20

Renal Corpuscle

1.6K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
1.6K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

12.3K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
12.3K
Reporter Genes02:11

Reporter Genes

11.1K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discordant Neuron-Specific Enolase and Neurologic Outcomes in Out-of-Hospital Cardiac Arrest: A Decade-Long Analysis.

Resuscitation·2026
Same author

Core-shell porous carbon hosts with spatially regulated lithium affinity for inward lithium deposition.

Nanoscale horizons·2026
Same author

Biomechanical analysis in hip joints according to sagittal pelvic tilt in non-ambulatory flaccid neuromuscular scoliosis: a finite element study.

Spine deformity·2026
Same author

Development and external validation of an explainable machine learning model for predicting chronic kidney disease progression in the Korean population.

Kidney research and clinical practice·2026
Same author

A comprehensive approach to elucidating the pathophysiology of kidney fibrosis based on extracellular vesicle proteomics.

Frontiers in physiology·2026
Same author

Stage-Specific Progression of Cardiovascular-Kidney-Metabolic Health and Mortality in a Nationwide Cohort of East Asian Adults.

Clinical journal of the American Society of Nephrology : CJASN·2026

Related Experiment Video

Updated: May 23, 2025

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.5K

Identification of conserved gene expression changes across common glomerular diseases by spatial transcriptomics.

Jeong Min Cho1,2, Minji Kang3, Sehoon Park4

  • 1Department of Translational Medicine, Seoul National University College of Medicine, Seoul, Korea.

Journal of Nephrology
|March 11, 2025
PubMed
Summary

Spatial transcriptomics revealed common downregulated genes in glomerular diseases, including JUN and FOS, offering insights into kidney disease mechanisms and potential therapies.

Keywords:
Digital spatial profilingGene expression profilingGlomerular diseaseGlomerulonephritisKidney biopsySpatial transcriptomic

More Related Videos

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

3.8K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Related Experiment Videos

Last Updated: May 23, 2025

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.5K
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

3.8K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Area of Science:

  • Nephrology
  • Genomics
  • Molecular Biology

Background:

  • Glomerular diseases share common gene expression pathways.
  • Analysis of glomerular-specific gene expression profiles across diverse glomerular diseases is crucial.

Purpose of the Study:

  • To analyze glomerular-specific gene expression profiles in various glomerular diseases.
  • To identify common differentially expressed genes across glomerular diseases.

Main Methods:

  • Spatial transcriptomic profiling of kidney biopsy specimens from controls and patients with five glomerular diseases.
  • Utilized GeoMx Digital Spatial Profiler for gene expression analysis.
  • Identified common differentially expressed genes and performed Gene Ontology annotation.

Main Results:

  • 35 genes were consistently downregulated in glomeruli across all studied glomerular diseases compared to controls.
  • Downregulated genes, including JUN and FOS, are associated with DNA-binding transcription factor activity.
  • Biological processes affected include response to lipids, steroid hormones, and cell cycle regulation.
  • Reduced expression of JUN, ZFP36, and KLF9 was confirmed in intraglomerular cells.

Conclusions:

  • Spatial transcriptomic analysis identified common differentially expressed genes in glomerular diseases.
  • These findings provide insights into the molecular mechanisms of glomerular diseases.
  • Potential for novel assessment and therapeutic strategies for kidney diseases.