Related Experiment Video
Updated: Jun 30, 2026
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
A programmable multichannel chemiluminescence immunoassay sensor for automatic quantification of the total amount of
Yakun Shi1, Huiyuan Lang2, Jin Ye3
1College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; Academy of National Food and Strategic Reserves Administration, NFSRA Key Laboratory of Grain and oil quality and safety, Beijing 100037, China.
Abstract:
A programmable multichannel chemiluminescence immunoassay sensor was developed based on the biotin-streptavidin system for the automatic quantification of the total amount of fumonisin B1, B2 and B3 in maize samples. First, the key parameters, such as the reaction time and reagent dosage were optimized to improve the sensitivity. All reagents were subsequently added to the corresponding wells of the reagent strips, which were further sealed to create a total fumonisin quantification kit. Finally, six samples were automatically and simultaneously analyzed within 20 min with the aid of a fully automated mycotoxin immunoassay analyzer. The limit of detection of this method is 16.32 μg/kg, with cross-reactivity for eight common mycotoxins being less than 0.66 %, and the spiking recovery rates ranging from 89.7 % to 103.7 %. In conclusion, the proposed programmable multichannel chemiluminescent immunoassay sensor provides a reliable, sensitive and practical detection tool for automated and high-throughput detection of FBs.
