Anti-inflammatory and antibacterial hydrogel based on a polymerizable ionic liquid
J A Romero-Antolín1, N Gómez-Cerezo1, M Manzano2
1Department of Chemistry in Pharmaceutical Sciences, School of Pharmacy, Institute Hospital 12 de Octubre (Imas12), Universidad Complutense de Madrid (UCM), 28040, Spain.
Acta Biomaterialia
|March 11, 2025
Summary
This study introduces a novel hydrogel for wound healing, combining natural polymers with a salicylate-releasing ionic liquid. The hydrogel promotes tissue repair and exhibits potent antibacterial activity against common wound pathogens.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Skin-infected wounds pose significant challenges due to inflammation and increasing bacterial resistance.
- Natural polymer-based hydrogels offer advantages for wound healing due to their biocompatibility and resemblance to native tissues.
Purpose of the Study:
- To develop a dual-action hydrogel with anti-inflammatory and antibacterial properties for wound treatment.
- To utilize a methacrylated gelatin matrix combined with a salicylate-anion-exchanged polymerizable ionic liquid (PIL).
Main Methods:
- Fabrication of a dual-action hydrogel incorporating methacrylated gelatin and a salicylate-functionalized PIL.
- Evaluation of cell attachment and colonization using mouse endothelial fibroblasts.
- Assessment of anti-inflammatory effects via flow cytometry and quantification of cytokines (TNF-α, IL-6, IL-10).
- Testing of antibacterial activity against Staphylococcus aureus and Escherichia coli.
Main Results:
- The hydrogel supported cell attachment and colonization.
- Demonstrated promotion of macrophage differentiation to an M2 (reparative) phenotype.
- Showed inhibition of pro-inflammatory cytokines (TNF-α, IL-6) and a relative increase in IL-10.
- Exhibited significant antibacterial activity against both Gram-positive and Gram-negative bacteria.
Conclusions:
- The developed hydrogel is biocompatible and possesses both anti-inflammatory and antibacterial properties.
- It effectively promotes a reparative cellular environment and combats bacterial infections.
- This novel hydrogel represents a promising candidate for advanced wound dressing applications.
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