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Low-cost and automated magnetic bead-based DNA data writing via digital microfluidics
Mengdi Bao1, Brett Herdendorf1, Gemma Mendonsa1
1Seagate Technology LLC, 1280 Disc Dr, Shakopee, MN 55379, USA. mengdi.bao@seagate.com.
Lab on a Chip
|March 12, 2025
Summary
This study introduces a digital microfluidics platform for automated DNA data storage writing. It uses DNAzymes for cost-effective, enzyme-free ligation, making DNA data storage more practical.
Area of Science:
- Biotechnology
- Data Storage
- Microfluidics
Background:
- Exponential data growth strains conventional storage.
- DNA data storage offers high density and durability.
- Current DNA writing methods are expensive and manual.
Purpose of the Study:
- Develop a programmable, cost-effective, automated DNA data writing process.
- Reduce the cost and complexity of DNA data storage.
- Enhance the scalability and commercial viability of DNA data storage.
Main Methods:
- Integrated digital microfluidics (DMF) with E47 DNAzyme ligation chemistry.
- Utilized pre-synthesized single-stranded DNA building blocks.
- Developed a DNAzyme-cleavage-assisted bead purification assay for on-platform ligation.
Main Results:
- Demonstrated an automated DNA writing process with reduced reagent input.
- Achieved enzyme-free, room-temperature ligation using DNAzymes.
- Reduced the number of required reservoirs due to high ligation specificity.
Conclusions:
- The DMF platform offers a cost-effective and automated solution for DNA data writing.
- This advancement simplifies DNA data storage systems.
- The technology enhances the practicality and scalability for commercial DNA data storage applications.
