Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Liquid storage of bone marrow.

A Mangalik, W A Robinson, C Drebing

    Experimental Hematology
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cutaneous Infections Caused by Pseudomonas aeruginosa. Clinical Presentation, Diagnosis and Treatment.

    Actas dermo-sifiliograficas·2026
    Same author

    Ex Vivo Confocal Laser Scanning Microscopy for Real-Time Pattern and Single-Cell Analysis in Inflammatory Skin Diseases.

    Experimental dermatology·2025
    Same author

    Precision at the Cutting Edge: Ex Vivo Confocal Microscopy for Perioperative Tumour Thickness Assessment in Melanoma.

    Experimental dermatology·2025
    Same author

    [Digital pathology using ex vivo confocal laser scanning microscopy].

    Dermatologie (Heidelberg, Germany)·2025
    Same author

    Incretin-responsive human pancreatic adipose tissue organoids: A functional model for fatty pancreas research.

    Molecular metabolism·2024
    Same author

    A literature review and expert consensus statement on diagnostics in suspected metal implant allergy.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2024

    Nonfrozen autologous bone marrow rescue can treat refractory neoplasms. Shorter storage of bone marrow cells before reinfusion improves hematological recovery after chemotherapy.

    Area of Science:

    • Oncology
    • Hematology
    • Cellular Biology

    Background:

    • Refractory nonhematological neoplasms require intensive treatment strategies.
    • Autologous bone marrow rescue is a critical component of high-dose chemotherapy.
    • Optimizing bone marrow cell viability during storage is essential for successful engraftment.

    Purpose of the Study:

    • To evaluate the feasibility of nonfrozen autologous bone marrow rescue for refractory nonhematological neoplasms.
    • To assess the impact of short-term in vitro storage on bone marrow cell viability, specifically Colony-Forming Units-Culture (CFU-C).
    • To determine the relationship between stored bone marrow cell dose and hematological recovery post-chemotherapy.

    Main Methods:

    • Patients with refractory nonhematological neoplasms received nonfrozen autologous bone marrow rescue.

    Related Experiment Videos

  • Bone marrow cells were stored in vitro at 4°C or 10°C for up to five days.
  • Colony-Forming Units-Culture (CFU-C) were quantified to assess cell viability.
  • Hematological recovery parameters were monitored post-chemotherapy and reinfusion.

    • Main Results:

    • In vitro storage of bone marrow cells at 4°C or 10°C for up to five days resulted in minor loss of CFU-C.
    • A gradual decline in CFU-C was observed with increasing storage duration.
    • Faster hematological recovery was associated with higher doses of infused CFU-C.
    • Shorter storage durations prior to reinfusion correlated with improved hematological recovery.

    Conclusions:

    • Nonfrozen autologous bone marrow rescue is a viable treatment option for refractory nonhematological neoplasms.
    • Short-term storage (up to five days) of autologous bone marrow cells at controlled temperatures (4°C or 10°C) is feasible with minimal loss of CFU-C.
    • Minimizing storage duration is recommended to optimize hematological recovery following high-dose chemotherapy and bone marrow reinfusion.