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Development of Immunocompetence01:22

Development of Immunocompetence

The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...

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Changes in rat plasma proteomes during the first week after birth.

Lilong Wei1, Zheng-Guang Guo2, Jing Wei3

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Newborn rat plasma proteomes change significantly within the first week of life, with distinct protein level alterations impacting development and survival. This study reveals key protein changes in early Wistar-rat development.

Keywords:
LC-MS/MSdevelopmentlabel-free quantificationorgan developmentplasma proteome

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Area of Science:

  • Proteomics
  • Developmental Biology
  • Animal Physiology

Background:

  • Blood plasma composition reflects physiological states due to secreted and leaked substances from tissues and cells.
  • Understanding plasma changes is crucial for monitoring body state and development.
  • Early life stages are critical for physiological adaptation and survival.

Purpose of the Study:

  • To investigate plasma proteome changes in Wistar rats during the transition from newborn to the first week of life.
  • To identify specific proteins and pathways that are altered during early postnatal development.
  • To establish a baseline for studying developmental changes in rat plasma proteomes.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for plasma proteomic analysis.
  • Label-free quantification was used to compare protein abundance between newborn and first-week rat plasma.
  • Differential protein cluster analysis and Ingenuity Pathway analysis (IPA) were utilized for data interpretation.

Main Results:

  • A total of 639 proteins were identified, with 570 used for quantitative analysis.
  • Significant changes were observed: 42 proteins increased and 17 proteins decreased in first-week rats compared to newborns.
  • Enhanced pathways included LXR/RXR Activation, DHCR24 Signaling, Acute Phase Response, and Reactive Oxygen Species Detoxification.

Conclusions:

  • Plasma proteomes of Wistar rats are distinct between newborn and first-week stages, indicating significant developmental shifts.
  • The identified proteomic changes likely facilitate the adaptation and survival of newborn rats.
  • This research provides the first LC-MS/MS-based analysis of newborn rat plasma proteomes, offering valuable insights into animal development.