Topography of brain metabolites: rhesus monkey, goat, and cat

This report describes the detailed topographic distribution of metabolites (ATP, phosphocreatine, lactate, glycogen, and glucose) in the brains of three large animal species: rhesus monkey, goat, and cat. The findings demonstrated that adequate fixation of tissue metabolites can be achieved for most brain structures in those animals using in situ freezing. This method, which enables studies of metabolite concentrations in hemispheral and deep brain structures in large animals, can be used to examine correlations between changes in brain metabolism and brain injury in a variety of models of neurologic disease.