Related Experiment Video
Updated: May 23, 2025
08:29
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
10.0K
Peptides: potential delivery systems for mRNA
Huiting Liang1, Yun Xing2, Kexin Wang3
1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory Shenzhen 518118 China yapi_zhang@163.com.
RSC Chemical Biology
|March 12, 2025
Summary
Peptide carriers show promise for delivering messenger RNA (mRNA) therapies, overcoming challenges in stability and cell entry. This review analyzes peptide-based systems for improved mRNA drug development.
Area of Science:
- Biotechnology
- Drug Delivery
- Molecular Biology
Background:
- Messenger RNA (mRNA) therapies offer broad applications including protein replacement, gene editing, and vaccines.
- Key challenges in mRNA therapy development include enhancing stability, translational efficiency, and reducing immunogenicity.
- Efficient and safe delivery of mRNA into target cells is a critical bottleneck due to its molecular size and electronegativity.
Purpose of the Study:
- To systematically analyze peptide-based systems for mRNA delivery.
- To explore construction strategies and mechanisms of action for peptide mRNA carriers.
- To identify limitations and challenges in the application of peptide carriers for mRNA drug development.
Main Methods:
- Review of existing literature on peptide-based mRNA delivery systems.
- Analysis of nanocarrier strategies, focusing on peptides.
- Examination of cell permeability, biocompatibility, and preparation ease of peptide carriers.
Main Results:
- Peptides exhibit high cell membrane permeability and good biocompatibility, making them promising mRNA carriers.
- Various peptide-based strategies are being explored for constructing effective mRNA delivery systems.
- Challenges remain in optimizing peptide carrier design for efficient and safe mRNA delivery.
Conclusions:
- Peptide carriers present a viable strategy for overcoming current mRNA delivery challenges.
- Further research and optimization of peptide carriers are crucial for advancing mRNA-based therapeutics.
- This review aims to guide the design and application of peptide carriers in future mRNA drug development.
More Related Videos
Related Concept Videos
02:22Regulated mRNA Transport
6.2K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.2K
02:51mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K
02:31Nuclear Export of mRNA
7.5K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.5K
01:25Nucleic Acid Structure
5.9K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
5.9K
02:43Nucleic Acids
43.3K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.3K
02:27Nonsense-mediated mRNA Decay
10.4K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.4K