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Spheroids from Epithelial and Mesenchymal Cell Phenotypes as Building Blocks in Bioprinting (Review)

D P Revokatova¹, P I Koteneva², N V Kosheleva³

¹Junior Researcher, Laboratory of Clinical Smart- and Nanotechnologies, Institute of Regenerative Medicine; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8/2 Trubetskaya St., Moscow, 119991, Russia.

Sovremennye Tekhnologii V Meditsine

|March 12, 2025

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View abstract on PubMed

Summary

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Materials Engineering

Wearable Materials

Spheroids From Epithelial And Mesenchymal Cell Phenotypes As Building Blocks In Bioprinting (review)

This summary is machine-generated.

Understanding the mechanical properties of epithelial and mesenchymal spheroids is crucial for advancing 3D bioprinting in regenerative medicine. This review compares their properties and bioprinting applications.

Area of Science:

Biomaterials Science
Regenerative Medicine
Cell Biology

Background:

Tissues comprise epithelial and mesenchymal cells, vital for barrier function, metabolism, support, and remodeling.
Cellular interactions are key for body integrity; tissue engineering aims to replicate this with artificial constructs.
Three-dimensional (3D) bioprinting utilizes cellular spheroids as building blocks for engineered tissues.

Purpose of the Study:

To review and compare the mechanical properties of epithelial and mesenchymal spheroids.
To examine co-culturing methods for regenerative medicine applications.
To analyze spheroid utilization in bioprinting for high-quality tissue construct development.

Main Methods:

Literature review of studies on spheroid mechanical properties.
Analysis of co-culturing techniques for epithelial and mesenchymal cells.
Evaluation of bioprinting strategies employing spheroids.

Main Results:

Significant differences exist in the mechanical properties of epithelial versus mesenchymal spheroids.
Spheroid mechanical properties influence cell migration, fusion, and construct morphology.
Co-culturing and specific bioprinting methods are critical for successful tissue engineering.

Conclusions:

Precise understanding of spheroid mechanics is essential for optimizing 3D bioprinting.
Tailoring bioprinting approaches based on cell type properties can enhance tissue construct quality.
This knowledge is vital for advancing regenerative medicine and creating functional artificial tissues.

Keywords:

bioprinting epithelial and mesenchymal cells mechanical properties of spheroids spheroids tissue engineering

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