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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Updated: May 22, 2025

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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Genomic and transcriptomic sequencing in prostate cancer.

Safiullah Rifai1, Azimullah Rifai1, Xiaolei Shi1,2

  • 1University of Maryland Greenebaum Comprehensive Cancer Center.

Current Opinion in Oncology
|March 12, 2025
PubMed
Summary

Next-generation sequencing advances prostate cancer characterization by identifying mutations and guiding treatment. RNA sequencing and gene classifiers are improving diagnosis and risk assessment for better patient outcomes.

Keywords:
RNA-seqdecipher scoregenomeprostate cancersequencingtranscriptomewhole exome sequencingwhole genome sequencing

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Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Genomic and transcriptomic sequencing technologies have transformed prostate cancer research.
  • Understanding the molecular landscape is crucial for effective prostate cancer management.

Purpose of the Study:

  • To review the current and evolving applications of next-generation sequencing in prostate cancer.
  • To highlight how these technologies aid in molecular characterization and treatment strategies.

Main Methods:

  • Review of genomic and transcriptomic sequencing methodologies.
  • Analysis of applications in identifying mutations, structural variations, and gene expression.
  • Integration of gene classifier platforms and mutation testing.

Main Results:

  • Sequencing identifies targetable mutations and structural variations in the prostate cancer genome.
  • RNA sequencing (RNA-seq) offers accurate quantitation and nucleotide-level resolution of the transcriptome.
  • Gene classifiers predict recurrence risk, and both somatic and germline mutation testing are increasingly recommended.

Conclusions:

  • Clinical integration of sequencing technologies enhances prostate cancer biology understanding.
  • Ongoing research and technological advancements promise improved prostate cancer treatments.