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Distance-Readout Paper-Based Microfluidic Chip with a DNA Hydrogel Valve for AFB1 Detection
Peng Chen1, Wenhao Han2, Yansheng Li2,3
1Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Robot Intelligent Laboratory of Traditional Chinese Medicine, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, P. R. China.
Analytical Chemistry
|March 12, 2025
Summary
A new paper-based microfluidic chip enables rapid, naked-eye detection of aflatoxin B1 (AFB1) in food. This sensitive method provides accurate results for on-site testing, ensuring food safety.
Area of Science:
- Food Science
- Analytical Chemistry
- Biotechnology
Background:
- Aflatoxin B1 (AFB1) is a toxic contaminant in food supplies, necessitating rapid and accurate detection methods.
- Current AFB1 detection methods often require complex instrumentation and trained personnel, limiting on-site application.
Purpose of the Study:
- To develop a simple, portable, and sensitive method for the quantitative detection of AFB1.
- To introduce a distance-readout paper-based microfluidic chip (DPMC) for visual AFB1 quantification.
Main Methods:
- Fabrication of a DPMC integrating a DNA hydrogel sensitive valve and a paper-based capillary channel.
- Utilizing the hydrogel valve to regulate liquid flow speed based on AFB1 concentration.
- Visual measurement of liquid travel distance in the capillary channel for quantitative AFB1 detection.
Main Results:
- The DPMC achieved quantitative detection of AFB1 in the range of 100–1000 pM with a detection limit of 17.64 pM.
- The method demonstrated high sensitivity and selectivity, allowing for naked-eye observation.
- Successful application in lotus seed samples with recovery rates between 85.2% and 118.4%.
Conclusions:
- The DPMC offers a rapid, portable, and sensitive visualization platform for on-site AFB1 detection.
- This technology enhances food safety by providing accessible and reliable aflatoxin testing.
- The DPMC eliminates the need for complex external equipment, simplifying field analysis.
