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DYF-5 regulates intraflagellar transport by affecting train turnaround
Wouter Mul1, Aniruddha Mitra1, Bram Prevo1
1Department of Physics and Astronomy, and LaserLaB, Vrije Universiteit Amsterdam, The Netherlands 1081HV.
Molecular Biology of the Cell
|March 12, 2025
Summary
The CILK1 homologue DYF-5 regulates intraflagellar transport (IFT) dynamics. Its absence disrupts IFT train turnarounds at the ciliary tip, causing cargo accumulation and altered transport.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Intraflagellar transport (IFT) is crucial for cilia function, coordinating cargo movement.
- CILK1 is a known regulator of IFT, but its precise mechanism remains elusive.
Purpose of the Study:
- To investigate the role of the CILK1 homologue DYF-5 in regulating IFT dynamics.
- To elucidate the mechanism by which DYF-5 influences IFT.
Main Methods:
- Fluorescence imaging in live *Caenorhabditis elegans* phasmid cilia.
- Single-molecule tracking to analyze IFT component dynamics.
Main Results:
- Absence of DYF-5 leads to IFT component accumulation at the ciliary tip.
- Kinesin-II distribution and velocity are altered, and retrograde IFT trains are significantly reduced.
- Reduced retrograde transport results in tip accumulation and base depletion of IFT components, impeding anterograde train assembly.
Conclusions:
- DYF-5 is essential for regulating IFT train turnarounds at the ciliary tip.
- DYF-5 dysfunction disrupts the balance of IFT, affecting both retrograde and anterograde transport.
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