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Supporting islet function in a PVDF membrane based macroencapsulation delivery device by solvent non-solvent casting

Denise F A de Bont¹, Sami G Mohammed¹, Rick H W de Vries¹

¹Cell Biology-Inspired Tissue Engineering (cBITE), MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.

|March 12, 2025

Summary

This study developed new porous membranes for islet transplantation, improving glucose diffusion and immune cell blocking. These membranes maintain islet viability and function, offering a promising alternative to lifelong insulin therapy for type 1 diabetes.

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Area of Science:

Biomaterials Science
Regenerative Medicine
Immunology

Background:

Type 1 diabetes (T1D) necessitates lifelong insulin therapy.
Clinical islet transplantation (CIT) is an alternative but limited by immunosuppression risks.
Macroencapsulation devices offer immunoprotection for transplanted cells.

Purpose of the Study:

To characterize and create porous polyvinylidene fluoride (PVDF) membrane devices for islet and beta-cell transplantation.
To improve glucose and insulin permeability while blocking immune cells.
To assess the viability and function of encapsulated human islets.

Main Methods:

Developed porous PVDF membranes incorporating polyvinyl-pyrrolidone (PVP) using solvent-non-solvent casting.
Investigated glucose diffusion and immune cell (macrophage) blocking capabilities.
Assessed human islet viability and glucose responsiveness within encapsulated devices.

Main Results:

PVDF/PVP membranes significantly increased glucose diffusion compared to PVDF alone.
Membranes effectively blocked human macrophages, preventing beta-cell damage.
Human islets encapsulated in PVDF/PVP devices showed high viability (92%) and improved glucose responsiveness (stimulation index 3.2).

Conclusions:

PVP incorporation tunes PVDF membrane diffusion characteristics for enhanced islet function.
These macroencapsulation devices maintain islet function comparable to free-floating islets.
The developed PVDF/PVP membranes offer a promising immunoprotective barrier for islet transplantation in T1D.