Related Experiment Video
Updated: Jul 28, 2026
The Polyvinyl Alcohol Sponge Model Implantation
Published on: April 18, 2012
Poly(vinyl alcohol)/Polycaprolactone Nanofiber Enriched with Lichenysin against Multidrug-Resistance Bacterial
Swati Yadav1, Dilip Kumar Arya1, Shubham Kanaujiya1
1Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, India.
Abstract:
Multidrug resistance (MDR) infectious wounds are a major concern due to drug resistance, leading to increased patient morbidity. Lichenysin (LCN), a lipopeptide and biosurfactant obtained from certain strains of Bacillus licheniformis, has demonstrated an excellent antimicrobial property. The present study focuses on the fabrication and comprehensive evaluation of LCN-incorporated poly(vinyl alcohol) (PVA)/polycaprolactone (PCL)-based nanofiber scaffolds using an electrospinning technique as a potential wound healing biomaterial for the treatment of MDR infectious wounds in diabetic rats. The LCN-loaded PVA-PCL nanofiber scaffolds were characterized for their physicochemical, antimicrobial, in vitro cell line on L-929, hemocompatibility, flow cytometry, in vivo infectious wound healing, and enzyme-linked immuno sorbent assay (ELISA). Morphological analysis via scanning electron microscopy (SEM) images confirmed smooth and porous nanofibers with diameters in the range 200-300 nm. Fourier transform infrared and X-ray diffraction (XRD) results demonstrated the structural integrity, chemical compatibility, and amorphous nature of developed scaffolds. The scaffolds loaded with LCN demonstrated excellent water retention, moderate biodegradability, and sustained release of LCN for up to 72 h. Mechanical characterization demonstrated a robust tensile strength conducive to wound healing applications. Antimicrobial activity against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) showed substantial antibacterial and antibiofilm activity. In vitro cell line studies showed enhanced cell adhesion, proliferation, migration, and viability, signifying the cytocompatibility of these scaffolds. In vivo studies demonstrated exceptional infectious wound healing potential in diabetic rats. These findings indicate that LCN-enriched PVA-PCL scaffolds hold significant potential as a therapeutic strategy for the treatment of MDR infectious wounds in diabetic rats through a multifaceted approach.
Insights
This study developed Lichenysin (LCN)-loaded poly(vinyl alcohol)/polycaprolactone nanofiber scaffolds for treating multidrug-resistant infectious wounds. These scaffolds show promise for enhanced wound healing in diabetic rats.
Area of Science:
- Biomaterials Science
- Infectious Diseases
- Regenerative Medicine
Background:
- Multidrug resistance (MDR) in wound infections increases patient morbidity.
- Lichenysin (LCN), a biosurfactant from *Bacillus licheniformis*, exhibits potent antimicrobial properties.
- Developing effective treatments for MDR wound infections, especially in diabetic patients, is critical.
Purpose of the Study:
- To fabricate and evaluate Lichenysin (LCN)-incorporated poly(vinyl alcohol)/polycaprolactone (PVA/PCL) nanofiber scaffolds.
- To assess the potential of these LCN-loaded scaffolds as a biomaterial for treating multidrug-resistant infectious wounds.
- To investigate the efficacy in a diabetic rat model.
Main Methods:
- Electrospinning was used to create LCN-loaded PVA/PCL nanofiber scaffolds.
- Scaffolds were characterized using SEM, FTIR, XRD, mechanical testing, and degradation studies.
- Antimicrobial activity, in vitro cytotoxicity (L-929 cells), hemocompatibility, and in vivo wound healing in diabetic rats were evaluated.
Main Results:
- SEM confirmed smooth, porous nanofibers (200-300 nm).
- Scaffolds exhibited good water retention, moderate biodegradability, and sustained LCN release up to 72 hours.
- Significant antibacterial and antibiofilm activity against *P. aeruginosa* and *S. aureus* was observed.
- In vitro studies showed enhanced cell viability, adhesion, proliferation, and migration.
- In vivo studies demonstrated accelerated infectious wound healing in diabetic rats.
Conclusions:
- LCN-enriched PVA/PCL nanofiber scaffolds show excellent physicochemical and mechanical properties for wound healing.
- These scaffolds possess significant antimicrobial, cytocompatible, and in vivo wound healing capabilities.
- The developed LCN-loaded scaffolds represent a promising therapeutic strategy for multidrug-resistant infectious wounds in diabetic models.
Related Concept Videos
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:25Clinical Significance of Antibiotic Resistance