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Updated: May 22, 2025

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Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
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Flow Cytometry for B-Cell Non-Hodgkin and Hodgkin Lymphomas
David C Gajzer1, Jonathan R Fromm1
1Department of Laboratory Medicine and Pathology, University of Washington, 825 Eastlake Ave E, Seattle, WA 98109, USA.
Cancers
|March 13, 2025
Summary
Flow cytometry rapidly classifies B-cell non-Hodgkin lymphoma (B-NHL) by assessing cellular antigen expression. This method aids disease classification and is influenced by B-cell-targeting therapies.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Multi-parametric flow cytometry offers rapid cellular antigen assessment for disease classification.
- B-cell non-Hodgkin lymphoma (B-NHL) diagnosis benefits from immunophenotypic analysis.
- Therapeutic effects on flow cytometry data require consideration.
Purpose of the Study:
- To detail B-cell non-Hodgkin lymphoma (B-NHL) classification using flow cytometry.
- To present established flow cytometry assays for specific lymphomas.
- To discuss evaluating reactive infiltrates in lymphomas.
Main Methods:
- Utilizing multi-parametric flow cytometry for immunophenotypic analysis.
- Developing and applying specific flow cytometry assay combinations.
- Analyzing cellular antigen expression patterns in B-NHL and Hodgkin lymphoma subtypes.
Main Results:
- Established flow cytometry assays effectively immunophenotype common B-NHL subtypes.
- Specific assay combinations are presented for classic Hodgkin lymphoma (CHL), nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), and T-cell/histocyte-rich large B-cell lymphoma (THRLBCL).
- The CHL assay is effective for primary mediastinal large B-cell lymphoma (PMLBCL) immunophenotyping.
Conclusions:
- Flow cytometry is a robust tool for B-NHL classification in clinical and research settings.
- Developed assays provide diagnostic utility for various B-NHL and Hodgkin lymphoma subtypes.
- Methodology for evaluating reactive infiltrates offers additional diagnostic information.
Keywords:
B-cell lymphomaB-cellsHodgkin lymphomaT-cell/histiocyte-rich large B-cell lymphomaclassic Hodgkin lymphomaclonalityflow cytometrylight chain restrictionnodular lymphocyte predominant Hodgkin lymphoma
