Related Experiment Video
Updated: May 22, 2025
08:10
Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
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Evaluation of Xpert® Carba-R Assay Performance from FecalSwab™ Samples
Nicla Giovacchini1,2, Chiara Chilleri1,2, Ilaria Baccani1,2
1Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy.
Diagnostics (Basel, Switzerland)
|March 13, 2025
Summary
The Xpert® Carba-R assay combined with FecalSwab™ offers an optimized method for detecting carbapenemase-producing Enterobacterales (CPE) carriers. This approach enhances diagnostic accuracy and efficiency for CPE screening.
Area of Science:
- Clinical microbiology
- Molecular diagnostics
- Infectious disease epidemiology
Background:
- Carbapenemase-producing Enterobacterales (CPE) pose a significant global health threat.
- Accurate and efficient detection of CPE carriers is crucial for infection control.
- Current diagnostic methods require optimization for widespread CPE screening.
Purpose of the Study:
- To evaluate the performance of the Xpert® Carba-R assay using FecalSwab™ for CPE carrier detection.
- To determine the optimal volume of FecalSwab™ for reliable results.
- To assess the impact of storage conditions on FecalSwab™ performance.
Main Methods:
- Real-Time PCR-based Xpert® Carba-R assay was used.
- FecalSwab™ liquid-based collection device was employed.
- Limit of detection and storage stability were assessed.
Main Results:
- 200 µL of FecalSwab™ medium yielded reliable Ct values and fewer invalid samples.
- Average limits of detection ranged from 4.7 × 10³ to 6.8 × 10³ CFU/mL.
- FecalSwab™ performed better after 16h at room temperature compared to 4°C.
Conclusions:
- FecalSwab™ is a suitable medium for single-step molecular and cultural CPE screening.
- This method optimizes CPE carrier detection, aiding infection control efforts.
- The combined approach enhances the efficiency and accuracy of CPE surveillance.
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