Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies

Mitochondria, as vital organelles, play a central role in subcellular research and biomedical innovation. Although functional nucleic acid (FNA) nanostructures have witnessed remarkable progress across numerous biological applications, strategies specifically tailored to target mitochondria for molecular imaging and therapeutic interventions remain scarce. This review delves into the latest advancements in leveraging FNA nanostructures for mitochondria-specific imaging and cancer therapy. Initially, we explore the creation of FNA-based biosensors localized to mitochondria, enabling the real-time detection and visualization of critical molecules essential for mitochondrial function. Subsequently, we examine developments in FNA nanostructures aimed at mitochondrial-targeted cancer treatments, including modular FNA nanodevices for the precise delivery of therapeutic agents and programmable FNA nanostructures for disrupting mitochondrial processes. Emphasis is placed on elucidating the chemical principles underlying the design of mitochondrial-specific FNA nanotechnology for diverse biomedical uses. Lastly, we address the unresolved challenges and outline prospective directions, with the goal of advancing the field and encouraging the creation of sophisticated FNA tools for both academic inquiry and clinical applications centered on mitochondria.