Related Experiment Video
Updated: Jun 29, 2026

Graphene Coatings for Biomedical Implants
Published on: March 1, 2013
Unveiling the Role of Fractionated Graphene Oxide in Nitric Oxide Scavenging
Grigoriy R Chermashentsev1, Ivan V Mikheev1, Daria-Mariia V Ratova1
1Department of Chemistry, Lomonosov Moscow State University, Moscow 119234, Russia.
Abstract:
The feasibility of saturating aqueous anoxic solutions with in situ-generated high-purity nitric oxide (NO) is shown herein. A methemoglobin assay estimated the average nitric oxide concentration to be ca. 20 ± 3 µM. Graphene oxide aqueous dispersions were prepared by ultrasound-assisted extra exfoliation. These dispersions, including unpurified (pristine) samples and samples purified from transition metal impurities (bulk) fractions (bulkGO) and (nano) separated fractions (nanoGO) in a range of 0.5 to 14 kDa were prepared with ppm level concentrations. A robust and reproducible chemiluminescence (CL) assay validated the interaction between graphene oxide and NO in a luminol-based system. The results showed a significant increase in NO scavenging activity within the bulkGO fractions to nanofractions ranging from 14 to 3.5 kDa. The different reaction pathways underlying the transformation of nitric oxide are being evaluated, focusing on understanding how its presence or absence affects these processes. Our kinetic model suggests a significant difference in nitric oxide regulation; nanoGO demonstrates an interception rate seventy-times higher than that achieved through CL quenching.
Related Concept Videos
Oxidative Cleavage of Alkenes: Ozonolysis
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Sample Preparation for Analysis: Advanced Techniques
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Catalysis
Microbial Bioremediation of Plastics

