Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Translational and epitranscriptomic regulation of seed germination in Arabidopsis thaliana genotypes with contrasting dormancy phenotypes.

Plant molecular biology·2025

Same author

Biochemical Analysis of Recombinant Pea Seed Coat-Specific Polyphenol Oxidase (<i>Pea</i>PPO) in Relation to Various Phenolic Substrates.

Journal of agricultural and food chemistry·2025

Same author

Genetic and transcriptomic analysis of lentil seed imbibition and dormancy in relation to its domestication.

The plant genome·2025

Same author

Paralogous Gene Recruitment in Multiple Families Constitutes Genetic Architecture and Robustness of Pod Dehiscence in Legumes.

Genome biology and evolution·2024

Same author

Evolutionary pathways to lower biomass allocation to the seed coat in crops: insights from allometric scaling.

The New phytologist·2024

Same author

Domestication has altered gene expression and secondary metabolites in pea seed coat.

The Plant journal : for cell and molecular biology·2024

Search research articles

Related Experiment Video

Updated: May 22, 2025

Collection and Identification of Pollen from Honey Bee Colonies

Collection and Identification of Pollen from Honey Bee Colonies

Published on: January 19, 2021

Modern Comprehensive Metabolomic Profiling of Pollen Using Various Analytical Techniques.

Petra Krejčí¹, Zbyněk Žingor¹, Jana Balarynová²

¹Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 771 46 Olomouc, Czech Republic.

Molecules (Basel, Switzerland)

|March 13, 2025

Summary

This study used advanced mass spectrometry techniques to analyze pollen's chemical makeup, revealing significant differences between major plant groups and aiding in taxonomic identification and understanding reproductive interactions.

Keywords:

Magnoliophyta Pinophyta atmospheric solids analysis probe infrared spectrometry mass spectrometry matrix-assisted laser desorption/ionization metabolite profiling metabolomics pollen secondary metabolite taxonomic differentiation ultra-high-performance liquid chromatography

More Related Videos

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 22, 2025

Collection and Identification of Pollen from Honey Bee Colonies

Collection and Identification of Pollen from Honey Bee Colonies

Published on: January 19, 2021

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

Plant biology and biochemistry
Analytical chemistry
Ecosystem science

Background:

Pollen's complex structure is vital for plant reproduction, genetic diversity, and ecosystem health.
Understanding pollen's chemical composition is crucial for studying plant-pollinator interactions, pollination, and allergies.

Purpose of the Study:

To perform comprehensive metabolite profiling of pollen using a multimodal analytical approach.
To investigate chemical differences between pollen from Magnoliophyta and Pinophyta.
To explore the utility of metabolomics data for taxonomic differentiation and understanding pollen's ecological roles.

Main Methods:

Fourier transform infrared spectrometry (FTIR) for initial metabolite class overview.
Atmospheric Solids Analysis Probe-MS (ASAP-MS) for characterizing the pollen's hydrophobic outer layer.
Matrix-Assisted Laser Desorption/Ionization (MALDI) and ultra-high-performance liquid chromatography-MS (UHPLC-MS) for detailed lipid, carbohydrate, and polyphenol profiling.

Main Results:

FTIR identified phenylpropanoid, lipid, and carbohydrate structures.
ASAP-MS revealed esters, phytosterols, and terpenoids in the pollen's outer layer.
UHPLC-MS demonstrated significant chemical distinctions between Magnoliophyta and Pinophyta pollen, with variations also noted within Magnoliophyta species.

Conclusions:

Multimodal mass spectrometry effectively differentiated pollen at the taxonomic level.
Metabolomics data provide insights into pollen's role in reproduction, ecological interactions, and potential applications.
The study highlights the chemical diversity of pollen and its ecological significance.