Native and Recombinant Yeast Producers of Lactic Acid: Characteristics and Perspectives

Lactic acid (LA) is a key chemical used in various industries, including food, pharmaceuticals, and bioplastics. Although traditionally produced using lactic acid bacteria, yeasts offer significant advantages, such as higher tolerance to acidic environments, a broader substrate range, and the potential for genetic and metabolic engineering. This review explores the potential use of Lachancea thermotolerans, Saccharomyces cerevisiae, Kluyveromyces marxianus, Kluyveromyces lactis, Candida utilis, and Pichia kudriavzevii as LA producers, highlighting their unique characteristics and industrial applications. S. cerevisiae stands out due to its robust genetic toolkit and acid tolerance, while K. marxianus offers thermotolerance and the efficient utilization of lactose and pentoses, making it ideal for high-temperature fermentations. K. lactis is particularly suited for valorizing dairy by-products like whey, P. kudriavzevii exhibits high tolerance to multiple stresses, while C. utilis demonstrates superior resilience to lignocellulosic inhibitors, enabling its use in biorefineries. Key challenges, including enhancing LA tolerance and optimizing metabolic pathways, are addressed through strategies like heterologous lactate dehydrogenase (LDH) expression, redox balance modification, and adaptive laboratory evolution. The review also discusses industrial applications, particularly in the context of circular economy approaches, where yeasts can convert waste streams into high-value LA. Future research should focus on integrating yeasts into scalable, sustainable bioprocesses to meet the growing demand for renewable and biodegradable materials.