A Broad-Spectrum Monoclonal Antibody-Based Heterologous ic-ELISA for the Detection of Multiple Pyrethroids in Water, Milk, Celery, and Leek
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View abstract on PubMed
Summary
This summary is machine-generated.A new monoclonal antibody (mAb) enables a sensitive ELISA test for detecting common pyrethroid insecticide residues in food and environmental samples. This rapid, cost-effective method aids in monitoring pyrethroid contamination.
Area Of Science
- Agricultural Science
- Environmental Science
- Analytical Chemistry
Background
- Pyrethroids are widely used insecticides in agriculture, public health, and consumer products.
- Monitoring pyrethroid residues in the environment and food is crucial for safety.
- Existing detection methods can be time-consuming and expensive.
Purpose Of The Study
- To develop a broad-spectrum monoclonal antibody (mAb) for pyrethroid detection.
- To establish a sensitive and rapid immunoassay for pyrethroid residue analysis.
- To validate the immunoassay against established analytical techniques.
Main Methods
- Production of a broad-spectrum monoclonal antibody (mAb), CL/CN-1D2.
- Development of a heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using the mAb.
- Validation of the ic-ELISA in various matrices (water, milk, celery, leek) and comparison with Gas Chromatography (GC).
Main Results
- The mAb CL/CN-1D2 showed varying IC50 values for eight different pyrethroids.
- The developed ic-ELISA demonstrated high sensitivity with limits of detection (LOD) ranging from 24.4 to 152.2 μg/kg.
- The assay exhibited good accuracy (65.1%–112.4% recovery) and precision (CV < 15%).
- A strong correlation (R² = 0.9945) was observed between ic-ELISA and GC results.
Conclusions
- The mAb CL/CN-1D2 is effective for broad-spectrum pyrethroid detection.
- The developed ic-ELISA is a reliable, rapid, and cost-effective tool for screening pyrethroid residues.
- This immunoassay can be utilized for routine monitoring in environmental and food safety applications.
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